A total of 133 isolates of yeast-like fungi was tested for sensitivity to seven different antifungal agents. The yeast-like fungi tested were isolated from the milk from normal or mastitic bovine quaters or from bovine feces. They were 5 Candida albicans (C. albicans) isolates, 63 C. krusei, 27 C. tropcalis, 5 C. parapsilosis, 10 Torulopsis glabrata, 6 Rhodotorula sp., 6 Hansenula sp. and 1 Pichia sp. isolate. The antifungal agents tested were nystatin, griseofulvin, cycloheximide, 5-fluorocytosine, miconazol, clotrimazole and tolnaftate.
In general, clotrimazole, miconazol and 5-fluorocytosine were more effective in antifungal activity in vitro against the test organisms than the rest of the agents tested. However, some of the isolates showed higher resistance to certain antifungal agents compared to the other isolates of the some species. They were: 1 C. albicans isolate to 5-fluorocytosine; 1 C. albicans to 5-fluorocytosine, miconazol and clotrimazole; 1 C. krusei to 5-fluorocytosine and cycloheximide; and 11 C. tropicalis isolates to cycloheximide.
The minimum inhibitory concentrations(MIC) of clotrimazole were 12.5㎍/㎖ or lower for all isolates tested except one C. albicans isolate, for which MIC of the drug was 100㎍/㎖. On the other hand, the MIC’s of cycloheximide were 6.5㎍/㎖ or lower for all isolates except the following; a11 isolates of C. albicans (100㎍/㎖), C. pseudotropicalis (200㎍/㎖) and Rkodotorula sp. (25-50㎍/㎖), 11 C. tropicalis isolates (100㎍/㎖) and 1 C. krusei isolate (200㎍/㎖).
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